3S0H
The crystal structure of the periplasmic domain of Helicobacter pylori MotB (residues 90-256).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Detector technology | CCD |
Collection date | 2009-04-08 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.87 |
Spacegroup name | P 43 |
Unit cell lengths | 72.972, 72.972, 127.178 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.100 |
R-factor | 0.1841 |
Rwork | 0.181 |
R-free | 0.23610 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.422 |
Data scaling software | SCALA (3.3.1) |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 51.571 | 51.571 | 2.210 |
High resolution limit [Å] | 2.100 | 6.640 | 2.100 |
Rmerge | 0.098 | 0.050 | 0.343 |
Total number of observations | 3055 | 14202 | |
Number of reflections | 37792 | ||
<I/σ(I)> | 6.8 | 11.4 | 2.2 |
Completeness [%] | 97.5 | 92.3 | 98.8 |
Redundancy | 2.6 | 2.7 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |