3S0G
Apis mellifera OBP 14 double mutant Gln44Cys, His97Cys
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-09 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 63.790, 72.990, 42.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.100 - 1.850 |
| R-factor | 0.2211 |
| Rwork | 0.221 |
| R-free | 0.22780 |
| Structure solution method | MIR |
| Starting model (for MR) | 3s0f |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.370 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.9.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.600 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.074 | 0.470 |
| Number of reflections | 17250 | |
| <I/σ(I)> | 16.1 | 4 |
| Completeness [%] | 99.5 | 99.9 |
| Redundancy | 4.7 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 1.6 M sodium phosphate monobasic, 1.6 M potassium phosphate dibasic, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
