3S0F

Apis mellifera OBP14 native apo, crystal form 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-03-11
Detector	MAR scanner 345 mm plate
Spacegroup name	P 21 21 2
Unit cell lengths	64.080, 75.380, 42.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.690 - 2.030
R-factor	0.1956
Rwork	0.194
R-free	0.21840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3s0a
RMSD bond length	0.010
RMSD bond angle	1.240
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER (2.9.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.200	2.080
High resolution limit [Å]	2.030	2.030
Rmerge	0.029	0.080
Number of reflections	13308
<I/σ(I)>	26.4	12
Completeness [%]	96.5	73.6
Redundancy	2.8	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	30% PEG4000, 10 mM imidazole-malate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K