3S0F
Apis mellifera OBP14 native apo, crystal form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | Cu FINE FOCUS |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-03-11 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 64.080, 75.380, 42.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.690 - 2.030 |
R-factor | 0.1956 |
Rwork | 0.194 |
R-free | 0.21840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3s0a |
RMSD bond length | 0.010 |
RMSD bond angle | 1.240 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | BUSTER (2.9.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.200 | 2.080 |
High resolution limit [Å] | 2.030 | 2.030 |
Rmerge | 0.029 | 0.080 |
Number of reflections | 13308 | |
<I/σ(I)> | 26.4 | 12 |
Completeness [%] | 96.5 | 73.6 |
Redundancy | 2.8 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 30% PEG4000, 10 mM imidazole-malate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |