3S0C
Transaldolase wt of Thermoplasma acidophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.91841 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 149.044, 172.024, 100.271 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.191 - 1.780 |
| R-factor | 0.1962 |
| Rwork | 0.195 |
| R-free | 0.22910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wx0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.108 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.199 | 1.830 | |
| High resolution limit [Å] | 1.780 | 7.960 | 1.780 |
| Rmerge | 0.039 | 0.016 | 0.438 |
| Number of reflections | 121135 | ||
| <I/σ(I)> | 21.32 | 57.7 | 2.61 |
| Completeness [%] | 98.5 | 85.4 | 96.3 |
| Redundancy | 4.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | hanging drop | 4.5 | 291 | PEG 6000, GOL, pH 4.5, hanging drop, temperature 291K |
