3S0B
Apis mellifera OBP14 in complex with the fluorescent probe 1-N-phenylnaphthylamine (NPN)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-08 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.380, 37.110, 89.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.880 - 1.220 |
| R-factor | 0.16783 |
| Rwork | 0.167 |
| R-free | 0.18347 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3s0a |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.513 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 1.250 |
| High resolution limit [Å] | 1.220 | 1.220 |
| Rmerge | 0.044 | 0.600 |
| Number of reflections | 32858 | |
| <I/σ(I)> | 18 | 2.2 |
| Completeness [%] | 99.4 | 99 |
| Redundancy | 4.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.8 | 293 | 1.8-1.9 M tri-sodium citrate, 25 mM CHES, pH 9.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
