3S06
The crystal structure of the periplasmic domain of Helicobacter pylori MotB (residues 97-256, P3121).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-08 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.87 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 70.714, 70.714, 143.421 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.1718 |
Rwork | 0.170 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.325 |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 61.240 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.066 |
Number of reflections | 38287 |
Completeness [%] | 98.1 |
Redundancy | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 100 mM Bicine pH 9.0, 2 M Ammonium Sulphate , VAPOR DIFFUSION, SITTING DROP, temperature 293K |