3S05

mEos2 Fluorescent Protein-Green Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	72.374, 105.800, 118.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.809 - 2.204
R-factor	0.1547
Rwork	0.151
R-free	0.21610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zux
RMSD bond length	0.024
RMSD bond angle	1.851
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall
Low resolution limit [Å]	78.874
High resolution limit [Å]	2.204
Number of reflections	46553
Completeness [%]	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	0.2M Magnesium Chloride hexahydrate, 0.1M Tris pH 8.5, 25% w/v Polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K