3S02
The crystal structure of the periplasmic domain of Helicobacter pylori MotB (residues 103-256)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.004 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 75.803, 75.803, 140.811 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.650 - 2.500 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.24080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.505 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.1) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.650 | |
| High resolution limit [Å] | 2.500 | |
| Rmerge | 0.090 | |
| Total number of observations | 10921 | |
| Number of reflections | 16761 | |
| <I/σ(I)> | 14.5 | |
| Completeness [%] | 99.9 | |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100 mM MES pH 6.5, 6% PEG 20k, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
