3RZX

Mouse importin alpha-Ku70 NLS peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-27
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.45859
Spacegroup name	P 21 21 21
Unit cell lengths	78.518, 89.996, 100.142
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.070 - 2.610
R-factor	0.17041
Rwork	0.168
R-free	0.22298
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pjn
RMSD bond length	0.025
RMSD bond angle	2.306
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.070	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	20974
Completeness [%]	99.2	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	Sodium Citrate, DTT, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K