3RZV
The Crystal Structure of a E280A Mutant of the Catalytic Domain of AMSH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-27 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 53.437, 53.437, 128.828 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.474 - 1.670 |
R-factor | 0.1812 |
Rwork | 0.180 |
R-free | 0.20500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rzv |
RMSD bond length | 0.006 |
RMSD bond angle | 0.993 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.710 |
High resolution limit [Å] | 1.670 | 4.560 | 1.670 |
Number of reflections | 22450 | ||
<I/σ(I)> | 28.3 | 36 | 3.7 |
Completeness [%] | 99.6 | 95.9 | 100 |
Redundancy | 12.1 | 11.1 | 12.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M MES, 15% PEG 6000, 5% MPD, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |