3RZ4
Hen egg-white lysozyme in HEPES buffer at pH 7.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2011-03-09 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.200, 77.200, 36.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 17.000 - 1.800 |
Rwork | 0.208 |
R-free | 0.22270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3fe0 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 17.000 | 1.850 | |
High resolution limit [Å] | 1.800 | 5.150 | 1.800 |
Number of reflections | 10731 | ||
<I/σ(I)> | 31.6 | 81.86 | 4.45 |
Completeness [%] | 97.0 | 98.9 | 86.7 |
Redundancy | 6.5 | 6.1 | 3.78 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 0.8M potassium sodium tartrate tetrahydrate, 0.1M HEPES sodium, pH 7.5, VAPOR DIFFUSION, HANGING DROP |