3RYL
Dimerization domain of Vibrio parahemolyticus VopL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-29 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.930, 90.490, 101.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 67.650 - 3.100 |
R-factor | 0.2459 |
Rwork | 0.240 |
R-free | 0.29360 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.512 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXS |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.650 | 3.200 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.122 | 0.426 |
Number of reflections | 10025 | |
<I/σ(I)> | 20.56 | 2.37 |
Completeness [%] | 99.5 | 99.9 |
Redundancy | 32.75 | 34.86 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 13-15% PEG3350, 0.2 M sodium fluoride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |