3RYB
Lactococcal OppA complexed with SLSQSLSQS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 70 |
| Detector technology | CCD |
| Collection date | 2009-09-25 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.379, 58.921, 61.404 |
| Unit cell angles | 100.66, 101.31, 103.45 |
Refinement procedure
| Resolution | 30.700 - 1.500 |
| R-factor | 0.1645 |
| Rwork | 0.163 |
| R-free | 0.20050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3drf chain A |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.328 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.730 | 4.240 | 1.610 |
| High resolution limit [Å] | 1.500 | 3.010 | 1.500 |
| Rmerge | 0.034 | 0.016 | 0.281 |
| Number of reflections | 83652 | 6567 | 14543 |
| <I/σ(I)> | 15.78 | 41.34 | 2.9 |
| Completeness [%] | 93.4 | 93.1 | 92.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.2M NACL, 0.1M NA-HEPES, 20% PEG 6000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
