3RY4

1.5 Angstrom resolution structure of glycosylated fcgammariia (low-responder polymorphism)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2002-04-11
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 2
Unit cell lengths	79.343, 100.407, 27.937
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.840 - 1.500
R-factor	0.203
Rwork	0.203
R-free	0.23000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fcg
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	35275
<I/σ(I)>	21.8	3.8
Completeness [%]	94.5	85.5
Redundancy	6.1	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.6		30% (W/V) PEG 4000, 0.2M AMMONIUM ACETATE, 0.1M SODIUM CITRATE, PH 5.60, VAPOR DIFFUSION, TEMPERATURE 291.0K