3RX6
Crystal structure of Polarity Suppression protein from Enterobacteria phage P4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR591 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-09-15 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.5418 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 148.750, 148.750, 63.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.039 - 2.039 |
R-factor | 0.1945 |
Rwork | 0.192 |
R-free | 0.21870 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.928 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.039 | 2.110 |
High resolution limit [Å] | 2.039 | 2.039 |
Rmerge | 0.058 | 0.058 |
Number of reflections | 22442 | |
<I/σ(I)> | 13.4 | 13.4 |
Completeness [%] | 98.5 | 98.5 |
Redundancy | 4.8 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 7.5% PEG 6000, 5% glycerol, 0.5mM DTT, 300mM NaCl, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |