3RX6

Crystal structure of Polarity Suppression protein from Enterobacteria phage P4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR591
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-09-15
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	I 4 2 2
Unit cell lengths	148.750, 148.750, 63.370
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.039 - 2.039
R-factor	0.1945
Rwork	0.192
R-free	0.21870
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.928
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.039	2.110
High resolution limit [Å]	2.039	2.039
Rmerge	0.058	0.058
Number of reflections	22442
<I/σ(I)>	13.4	13.4
Completeness [%]	98.5	98.5
Redundancy	4.8	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	7.5% PEG 6000, 5% glycerol, 0.5mM DTT, 300mM NaCl, 0.1M MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K