3RWA
Crystal structure of circular-permutated mKate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 70 |
Detector technology | CCD |
Collection date | 2011-04-20 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.2158 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 109.357, 93.280, 194.370 |
Unit cell angles | 90.00, 96.52, 90.00 |
Refinement procedure
Resolution | 38.311 - 1.670 |
R-factor | 0.1823 |
Rwork | 0.182 |
R-free | 0.21500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.231 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.730 |
High resolution limit [Å] | 1.670 | 3.600 | 1.670 |
Rmerge | 0.057 | 0.044 | 0.217 |
Number of reflections | 223214 | ||
<I/σ(I)> | 14.9 | ||
Completeness [%] | 99.2 | 99.8 | 93.4 |
Redundancy | 4 | 4.3 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 273 | 0.1M Tris-HCl pH 7.4, 0.2M MgCl2 and 18% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 273K |