3RVP
Structure of the CheY-BeF3 Complex with substitutions at 59 and 89: N59D and E89K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-07 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00882 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.487, 53.626, 160.416 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.580 - 2.404 |
R-factor | 0.1775 |
Rwork | 0.173 |
R-free | 0.21470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fqw |
RMSD bond length | 0.007 |
RMSD bond angle | 1.058 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 18144 | |
<I/σ(I)> | 22.3 | 2.57 |
Completeness [%] | 96.9 | 70.7 |
Redundancy | 6.4 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Ammonium Sulfate 2.6M, Tris 100mM pH 7.5, Glycerol 5% (v/v), MnCl2 20mM, BeCl2 1mM, NaF 10mM, 4.6mg/mL, VAPOR DIFFUSION, HANGING DROP, temperature 298K |