3RVN
Structure of the CheY-BeF3 Complex with substitutions at 59 and 89: N59D and E89Y
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-BM |
Synchrotron site | APS |
Beamline | 22-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-27 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97933 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.436, 53.613, 161.310 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.597 - 2.250 |
R-factor | 0.1883 |
Rwork | 0.186 |
R-free | 0.22520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fqw |
RMSD bond length | 0.007 |
RMSD bond angle | 1.110 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.290 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 22709 | |
<I/σ(I)> | 12.82 | 2.6 |
Completeness [%] | 99.8 | 99.9 |
Redundancy | 5 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.25 | 298 | Ammonium Sulfate 2.0M Tris 100mM pH 8.25 Glycerol 5% (v/v) MnCl2 20mM BeCl2 1mM NaF 10mM CheY 8.9mg/mL, VAPOR DIFFUSION, HANGING DROP, temperature 298K |