3RV3
Crystal structure of E.coli biotin carboxylase in complex with two ADP and one Mg ion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Detector technology | CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 171.357, 58.009, 85.236 |
| Unit cell angles | 90.00, 94.64, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.910 |
| R-factor | 0.2105 |
| Rwork | 0.208 |
| R-free | 0.26250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.351 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.117 | 0.085 | 0.371 |
| Number of reflections | 64727 | ||
| <I/σ(I)> | 7.2 | ||
| Completeness [%] | 99.9 | 99.9 | 99.7 |
| Redundancy | 6 | 6 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 295 | Bis-Tris, PEG3350, NH4Cl, n-octyl-beta-D-glucose, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
