3RV1

Crystal structure of the N-terminal and RNase III domains of K. polysporus Dcr1 E224Q mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	58.907, 96.405, 101.449
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.203 - 1.975
R-factor	0.1965
Rwork	0.194
R-free	0.23590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.947
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX (dev_538)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.970
Number of reflections	40348
Completeness [%]	97.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	200 mM L-proline, 10% PEG3350, 100 mM HEPES buffer pH 7.5, vapor diffusion, temperature 293K