3RV0

Crystal structure of K. polysporus Dcr1 without the C-terminal dsRBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	101.037, 112.974, 135.689
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.410 - 2.290
R-factor	0.1774
Rwork	0.175
R-free	0.21970
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	0.974
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB
Refinement software	PHENIX (dev_538)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.290
Number of reflections	69722
<I/σ(I)>	27.3
Completeness [%]	99.7
Redundancy	10

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	180 mM Triammonium citrate, 20% PEG3350, 10 mM Na-HEPES buffer pH 7.5, vapor diffusion, temperature 293K