3RUV

Crystal structure of Cpn-rls in complex with ATP analogue from Methanococcus maripaludis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-01
Detector	ADSC QUANTUM 315r
Spacegroup name	I 2 2 2
Unit cell lengths	161.019, 184.477, 184.785
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.813 - 2.242
R-factor	0.1733
Rwork	0.171
R-free	0.22040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kfb
RMSD bond length	0.008
RMSD bond angle	1.066
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.240
Number of reflections	131211
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	298	20% PEG3350, 0.2 M lithium sulfate, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K