3RUP
Crystal structure of E.coli biotin carboxylase in complex with two ADP and two Ca ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Detector technology | CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.180, 58.843, 85.083 |
| Unit cell angles | 90.00, 94.24, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.990 |
| R-factor | 0.1736 |
| Rwork | 0.171 |
| R-free | 0.22690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.312 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 84.820 |
| High resolution limit [Å] | 1.990 |
| Number of reflections | 56480 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | PEG3350, CaCl2, pH 8.5, vapor diffusion, sitting drop, temperature 295K |
