3RUM
New strategy to analyze structures of glycopeptide antibiotic-target complexes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Collection date | 2010-03-03 |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.833, 75.005, 90.778 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.416 - 1.851 |
| R-factor | 0.1785 |
| Rwork | 0.176 |
| R-free | 0.21910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1anf |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.6.2_432) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.420 |
| High resolution limit [Å] | 1.850 |
| Number of reflections | 33176 |
| Completeness [%] | 96.5 |
| Redundancy | 4.06 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 5% isopropanol, 2M ammonium sulfate, vapor diffusion, sitting drop, temperature 291K |
