3RUJ

Crystal Structure of N-terminal region of yeast Atg7

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-09
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0, 0.9795, 0.9798, 0.95
Spacegroup name	I 41
Unit cell lengths	112.846, 112.846, 102.248
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.260 - 2.100
R-factor	0.2292
Rwork	0.228
R-free	0.25410
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.319
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.262	2.180
High resolution limit [Å]	2.100	2.100
Number of reflections	31750
Completeness [%]	99.2	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	0.1M Na-Hepes pH 8.0, 1.5M LiSO4H2O, 0.01M spermidine tetrahydrochloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K