3RUH
Alternative analogs as viable substrates of UDP-hexose 4-epimerases
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2011-04-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 32 |
| Unit cell lengths | 76.612, 76.612, 220.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 73.530 - 2.880 |
| R-factor | 0.21259 |
| Rwork | 0.210 |
| R-free | 0.26845 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lu1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.153 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.880 |
| Number of reflections | 30694 |
| Completeness [%] | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 6.5 | 300 | 25% PEG 3350, 0.2 M Ammonium Sulfate, 200 mM Bis Tris Propane pH 6.5, microbatch under oil, temperature 300K |
