3RUG
Crystal structure of Valpha10-Vbeta8.1 NKT TCR in complex with CD1d-alphaglucosylceramide (C20:2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95453 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.140, 118.834, 108.125 |
Unit cell angles | 90.00, 110.24, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.320 |
High resolution limit [Å] | 2.200 | 6.600 | 2.200 |
Rmerge | 0.149 | 0.056 | 0.698 |
Number of reflections | 97190 | ||
<I/σ(I)> | 9.2 | 21.9 | 2.6 |
Completeness [%] | 99.9 | 99.7 | 99.7 |
Redundancy | 5.5 | 5.6 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 20% PEG 1500, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |