3RUB

CRYSTAL STRUCTURE OF THE UNACTIVATED FORM OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE FROM TOBACCO REFINED AT 2.0-ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	I 4 2 2
Unit cell lengths	148.700, 148.700, 137.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.171
RMSD bond length	0.019
RMSD bond angle	0.045 *
Refinement software	PROFFT

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.000 *	2.000 *
Rmerge	0.088 *	0.214 *
Number of reflections	43733 *
Completeness [%]	84.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.2 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	phosphate	50 (mM)
3	1	drop	EDTA	0.5 (mM)
4	1	drop	azide	0.1 (mM)
5	1	reservoir	phosphate	0.2 (M)
6	1	reservoir		0.3 (M)
7	1	reservoir	azide	1 (mM)