3RU5
Silver Metallated Hen Egg White Lysozyme at 1.35 A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | BRUKER IMUS MICROFOCUS |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-28 |
Detector | APEX II CCD |
Wavelength(s) | 1.54 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.798, 77.798, 37.629 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.550 - 1.350 |
R-factor | 0.19348 |
Rwork | 0.192 |
R-free | 0.21451 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3kam |
RMSD bond length | 0.008 |
RMSD bond angle | 1.175 |
Data reduction software | SAINT |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.550 |
High resolution limit [Å] | 1.350 |
Number of reflections | 25935 |
<I/σ(I)> | 32.84 |
Completeness [%] | 99.7 |
Redundancy | 18.08 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 293 | 5 mM silver(I) 3-hydroxybutyrate, 3% (w/v) sodium nitrate, 25% (v/v) ethylene glycol, pH 4.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |