3RT0
Crystal structure of PYL10-HAB1 complex in the absence of abscisic acid (ABA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-27 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.766, 83.503, 88.544 |
| Unit cell angles | 90.00, 97.20, 90.00 |
Refinement procedure
| Resolution | 32.499 - 2.113 |
| R-factor | 0.1748 |
| Rwork | 0.173 |
| R-free | 0.20670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rt2 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.074 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.3_473)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.399 | |
| Number of reflections | 58037 | |
| <I/σ(I)> | 4.16 | |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 100mM Tris, 21% PEG3350, 2% Dioxane , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
