3RSY
Cellobiose phosphorylase from Cellulomonas uda in complex with sulfate and glycerol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2007-11-03 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0015 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.100, 103.780, 99.150 |
Unit cell angles | 90.00, 96.57, 90.00 |
Refinement procedure
Resolution | 19.976 - 1.810 |
R-factor | 0.2038 |
Rwork | 0.202 |
R-free | 0.23830 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.537 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.976 | 1.860 |
High resolution limit [Å] | 1.810 | 1.810 |
Number of reflections | 148787 | |
Completeness [%] | 94.6 | 87.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 1.8 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |