3RSN
Crystal Structure of the N-terminal region of Human Ash2L
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-07 |
Detector | MAR 300 CCD |
Wavelength(s) | 0.97856 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 49.984, 49.984, 165.519 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.057 - 2.100 |
R-factor | 0.2171 |
Rwork | 0.213 |
R-free | 0.25390 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.117 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.074 | |
Number of reflections | 14752 | |
<I/σ(I)> | 73 | |
Completeness [%] | 99.4 | 97.9 |
Redundancy | 19 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 100 mM Tris-HCl pH8.5, 20% PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |