3RRU
X-ray crystal structure of the VHS domain of human TOM1-like protein, Northeast Structural Genomics Consortium Target HR3050E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-01-28 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97916 |
Spacegroup name | P 31 1 2 |
Unit cell lengths | 122.047, 122.047, 55.095 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.980 - 3.000 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.25200 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SnB (then SOLVE/RESOLVE) |
Refinement software | CNS (1.2 & XtalView) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.117 | 0.339 |
Number of reflections | 17498 | |
<I/σ(I)> | 6.9 | 1.61 |
Completeness [%] | 95.3 | 81.5 |
Redundancy | 1.9 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 291 | Protein solution: 10 mM Tris (pH 7.5), 100 mM sodium chloride, 5 mM DTT, and 0.02% NaN3. Reservoir solution: 100mM MES (pH 6), 40% PEG 8000, and 100mM Ammonium Sulfate, micro batch under oil, temperature 291K |