3RRK
Crystal structure of the cytoplasmic N-terminal domain of subunit I, homolog of subunit a, of V-ATPase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-05 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97926 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 76.320, 102.181, 139.062 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.620 - 2.640 |
R-factor | 0.193 |
Rwork | 0.190 |
R-free | 0.25500 |
Structure solution method | SAD |
Starting model (for MR) | SAD phasing |
RMSD bond length | 0.008 |
RMSD bond angle | 1.096 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.620 | 2.690 |
High resolution limit [Å] | 2.640 | 2.640 |
Rmerge | 0.049 | 0.469 |
Number of reflections | 16308 | |
<I/σ(I)> | 29.4 | 3.1 |
Completeness [%] | 98.1 | |
Redundancy | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 0.2 M lithium sulfate, 0.8 M sodium-potassium trtarate, 0.1 M CHES, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |