3RRH
Ternary Structure of the large fragment of Taq DNA polymerase bound to an abasic site and a ddTTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-10-15 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 108.305, 108.305, 90.151 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.897 - 1.800 |
| R-factor | 0.1681 |
| Rwork | 0.166 |
| R-free | 0.20060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3lwl |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.003 |
| Data scaling software | XDS |
| Phasing software | PHENIX (dev_613) |
| Refinement software | PHENIX ((phenix.refine: dev_613)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.790 |
| Rmerge | 0.097 | 1.200 |
| <I/σ(I)> | 15.23 | 2.12 |
| Completeness [%] | 99.5 | 96.9 |
| Redundancy | 9.95 | 9.82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.05 M Na cacodylate pH 6.5, 0.2 M NH4OAc, 0.01 M Mg(OAc)2, 28% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
