3RR7
Binary Structure of the large fragment of Taq DNA polymerase bound to an abasic site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-02-17 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 109.460, 109.460, 90.427 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.398 - 1.950 |
R-factor | 0.1951 |
Rwork | 0.193 |
R-free | 0.23180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3lwl |
RMSD bond length | 0.002 |
RMSD bond angle | 0.739 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX (dev_704) |
Refinement software | PHENIX ((phenix.refine: dev_704)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.109 | 0.882 |
<I/σ(I)> | 10.29 | 1.27 |
Completeness [%] | 98.9 | 93.6 |
Redundancy | 5.11 | 4.97 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M TRIS HCl pH 7.5, 0.2 M Magnesium Formate, 12% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |