3RQC
Crystal structure of the catalytic core of the 2-oxoacid dehydrogenase multienzyme complex from Thermoplasma acidophilum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9702 |
Spacegroup name | H 3 2 |
Unit cell lengths | 204.840, 204.840, 441.940 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.950 - 4.010 |
R-factor | 0.25672 |
Rwork | 0.253 |
R-free | 0.32773 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eaf |
RMSD bond length | 0.015 |
RMSD bond angle | 1.806 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | BALBES |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.950 | 4.140 |
High resolution limit [Å] | 4.010 | 4.000 |
Number of reflections | 28168 | |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 15% MPD, 0.2M NaCl, 0.1M Na Acetate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |