3RQB
Crystal Structure of Conserved Protein of Unknown Function with Hot dog Fold from Alicyclobacillus acidocaldarius
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 109.645, 109.645, 192.614 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.478 - 2.800 |
R-factor | 0.179 |
Rwork | 0.175 |
R-free | 0.25700 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.243 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.480 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 17458 | |
<I/σ(I)> | 6.4 | 2.4 |
Completeness [%] | 99.5 | 99.5 |
Redundancy | 8.1 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 0.2 M Calcium Acetate, 0.1 M Sodium Acetate:Acetic Acid pH 4.5 30% (v/v) PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 289K |