3RQ9
Structure of Tsi2, a Tse2-immunity protein from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792, 0.9075 |
| Spacegroup name | P 1 |
| Unit cell lengths | 27.807, 34.063, 37.650 |
| Unit cell angles | 110.48, 95.94, 95.82 |
Refinement procedure
| Resolution | 34.880 - 1.000 |
| R-factor | 0.14655 |
| Rwork | 0.145 |
| R-free | 0.17613 |
| Structure solution method | MAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0096) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.010 |
| High resolution limit [Å] | 0.990 | 0.990 |
| Rmerge | 0.035 | 0.197 |
| Number of reflections | 60521 | |
| <I/σ(I)> | 31.2 | 3.7 |
| Completeness [%] | 100.0 | 45 |
| Redundancy | 3.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 8% PEG 4000, 0.1M sodium acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
