3RPW
The crystal structure of an ABC transporter from Rhodopseudomonas palustris CGA009
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97923 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 80.711, 80.711, 109.650 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.500 - 1.650 |
R-factor | 0.1679 |
Rwork | 0.166 |
R-free | 0.19520 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.118 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.500 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.084 | 0.617 |
Number of reflections | 50234 | |
<I/σ(I)> | 36.3 | 2.8 |
Completeness [%] | 99.8 | 100 |
Redundancy | 6 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 2.4M Sodium Malonate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |