3RPM
Crystal structure of the first GH20 domain of a novel Beta-N-acetyl-hexosaminidase StrH from Streptococcus pneumoniae R6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97893 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 173.984, 173.984, 108.204 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.560 - 2.100 |
| R-factor | 0.19312 |
| Rwork | 0.192 |
| R-free | 0.22133 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.192 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.109 | 0.475 |
| Number of reflections | 209284 | |
| <I/σ(I)> | 21.3 | 3.7 |
| Completeness [%] | 98.2 | 86.7 |
| Redundancy | 10.9 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 289 | 30% PEG 4000, 0.2M ammonium acetate, 0.1M Sodium citrate tribasic dehydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
