3RPL
D-fructose 1,6-bisphosphatase class 2/sedoheptulose 1,7-bisphosphatase of Synechocystis sp. PCC 6803 in complex with FRUCTOSE-1,6-BISPHOSPHATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-08-14 |
Detector | OXFORD ONYX CCD |
Wavelength(s) | 1.5418 |
Spacegroup name | P 65 |
Unit cell lengths | 145.222, 145.222, 169.104 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.719 - 2.400 |
R-factor | 0.1683 |
Rwork | 0.166 |
R-free | 0.20660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3roj |
RMSD bond length | 0.011 |
RMSD bond angle | 1.188 |
Data reduction software | CrysalisPro |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.720 | 25.720 | 2.530 |
High resolution limit [Å] | 2.400 | 7.590 | 2.400 |
Rmerge | 0.131 | 0.028 | 0.609 |
Number of reflections | 78184 | ||
<I/σ(I)> | 12.4 | 35.4 | 2.2 |
Completeness [%] | 99.2 | 97.4 | 95.1 |
Redundancy | 6.4 | 6.5 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 100mM HEPES, 1.6M Ammonium Sulfate, 7-9% glycerol, 10mM FBP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |