3RPK
Structure of the Full-Length Major Pilin RrgB from Streptococcus pneumoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 84.370, 107.930, 142.450 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.470 - 2.800 |
R-factor | 0.2206 |
Rwork | 0.217 |
R-free | 0.28050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2x9w |
RMSD bond length | 0.010 |
RMSD bond angle | 1.320 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.8.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 30964 | |
<I/σ(I)> | 5.6 | 2.1 |
Completeness [%] | 95.5 | 97.5 |
Redundancy | 3 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 1.9M ammonium sulfate, 0.1M Tris-HCl, 100mM ammonium iodide, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |