3RPD
The structure of a B12-independent methionine synthase from Shewanella sp. W3-18-1 in complex with Selenomethionine.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.099, 89.371, 86.526 |
| Unit cell angles | 90.00, 92.45, 90.00 |
Refinement procedure
| Resolution | 36.500 - 1.500 |
| R-factor | 0.1649 |
| Rwork | 0.164 |
| R-free | 0.18900 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.425 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 4.070 | 1.500 |
| Rmerge | 0.088 | 0.071 | 0.425 |
| Number of reflections | 111443 | ||
| <I/σ(I)> | 8.1 | ||
| Completeness [%] | 99.2 | 98.3 | 98.8 |
| Redundancy | 4.4 | 4.4 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.1M Imidazole:HCl pH 8.0, 1.0M Sodium Citrate Tribasic, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
