3RP9
Crystal Structure of the apo MapK from Toxoplasma Gondii, 25.m01780 or TGME49_007820
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-21 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.508, 97.454, 48.374 |
| Unit cell angles | 90.00, 104.92, 90.00 |
Refinement procedure
| Resolution | 33.732 - 2.400 |
| R-factor | 0.2031 |
| Rwork | 0.201 |
| R-free | 0.23750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nie |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.223 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 33.732 | 40.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 6.370 | 2.350 |
| Rmerge | 0.055 | 0.023 | 0.550 |
| Number of reflections | 17378 | ||
| <I/σ(I)> | 12.9 | 2.41 | |
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 3.7 | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 25% PEG3350, 0.1 M ammonium sulfate, 0.1 M Bis-Tris, pH 5.5, 10 mM magnesium chloride, 10 mM TCEP, cryoprotectant: 15% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
