3ROT
Crystal structure of ABC sugar transporter (periplasmic sugar binding protein) from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.693, 117.860, 54.607 |
| Unit cell angles | 90.00, 107.39, 90.00 |
Refinement procedure
| Resolution | 43.230 - 1.910 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.25400 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.108 | 0.667 |
| Number of reflections | 44651 | |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 98.6 | 90.9 |
| Redundancy | 7.2 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 10% PEG 6000, 5% MPD, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
