3ROD
Methyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2008-03-06 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.663, 61.793, 74.342 |
| Unit cell angles | 106.57, 103.98, 104.14 |
Refinement procedure
| Resolution | 39.670 - 2.720 |
| R-factor | 0.208 |
| Rwork | 0.205 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iip |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.678 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.670 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.091 | |
| Number of reflections | 24960 | |
| <I/σ(I)> | 9.6 | 1.9 |
| Completeness [%] | 98.7 | 97.5 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% PEG3350, 0.1 M BIS-TRIS; OR 25- 27% PEG3350, 0.2 M NACL, 0.1M BIS-TRIS OR IMIDAZOLE, PH 5.5 OR 6.5, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |
