3RO8
Crystal structure of the catalytic domain of XynA1 from Paenibacillus sp. JDR-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2008-08-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.072, 93.172, 182.947 |
| Unit cell angles | 90.00, 99.96, 90.00 |
Refinement procedure
| Resolution | 42.610 - 1.340 |
| R-factor | 0.14421 |
| Rwork | 0.142 |
| R-free | 0.18546 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.179 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 179.610 |
| High resolution limit [Å] | 1.340 |
| Number of reflections | 602662 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 294.5 | 200mM HEPES, 100 mM MgCl2, 18% PEG3350, pH 7.1, VAPOR DIFFUSION, SITTING DROP, temperature 294.5K |
