3RO2
Structures of the LGN/NuMA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-05 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 91.305, 91.305, 178.376 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.140 - 2.300 |
| R-factor | 0.21753 |
| Rwork | 0.215 |
| R-free | 0.27091 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.004 |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.140 |
| High resolution limit [Å] | 2.300 |
| Number of reflections | 20308 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.02M MgCl2, 0.1M HEPES pH 7.5, 22% w/v polyacrylic acid 5100 buffer, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
