3RO2
Structures of the LGN/NuMA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-05 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 91.305, 91.305, 178.376 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.140 - 2.300 |
R-factor | 0.21753 |
Rwork | 0.215 |
R-free | 0.27091 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.004 |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 36.140 |
High resolution limit [Å] | 2.300 |
Number of reflections | 20308 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.02M MgCl2, 0.1M HEPES pH 7.5, 22% w/v polyacrylic acid 5100 buffer, VAPOR DIFFUSION, HANGING DROP, temperature 291K |