3RNV
Structure of the autocatalytic cysteine protease domain of potyvirus helper-component proteinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9796 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 98.806, 98.806, 65.747 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.000 - 2.000 |
| R-factor | 0.24751 |
| Rwork | 0.246 |
| R-free | 0.27411 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.051 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.073 | 0.528 |
| Number of reflections | 11250 | |
| <I/σ(I)> | 48.7 | 4.1 |
| Completeness [%] | 98.6 | 98.7 |
| Redundancy | 13.1 | 10.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 277 | 1M (NH4)2SO4, 4%(w/v) PEG3350, 0.1M Bis-Tris, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
